Human Caspase 1 ELISA Kit
Sensitivity
1.0 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : KE00231
Synonyms
CASP 1, CASP1, Caspase 1, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, P45
Validation Data Gallery
Product Information
KE00231 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Caspase 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Caspase 1. The assay recognizes human Caspase 1. An antibody specific for Caspase 1 has been pre-coated onto the microwells. The Caspase 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human Caspase 1 is added to detect the captured human Caspase 1 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Caspase 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 834 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 77%-92% |
| Cell cuture supernatants | 100% | 92%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 197.4 | 6.2 | 3.1 |
| 2 | 20 | 46.3 | 1.5 | 3.3 |
| 3 | 20 | 10.2 | 0.7 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 198.0 | 12.1 | 6.1 |
| 2 | 24 | 50.1 | 4.2 | 8.4 |
| 3 | 24 | 11.9 | 0.9 | 7.9 |
Background Information
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CASP 1, CASP1, Caspase 1, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, P45 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
