Human CYCS ELISA Kit
Validation Data Gallery
Product Information
KE00079 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CYCS ELISA kit is to be used to detect and quantify protein levels of endogenous CYCS. The assay recognizes human CYCS. An antibody specific for CYCS has been pre-coated onto the microwells. The CYCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CYCS is added to detect the captured CYCS protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CYCS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54205 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 96% | 74%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 545.4 | 21.6 | 4.0 |
| 2 | 20 | 1,398.4 | 51.0 | 3.6 |
| 3 | 20 | 2,467.2 | 119.3 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 513.0 | 45.9 | 9.0 |
| 2 | 24 | 1,370.1 | 90.9 | 6.6 |
| 3 | 24 | 2,503.1 | 97.8 | 3.9 |
Background Information
CYCS, Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy. This kit is designed to quantify the level of cytochrome c protein.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Free Radic Biol Med Glabridin Protects Against Paraquat-Induced Acute Lung Injury by Targeting ME1 to Mitigate Oxidative Stress, Mitochondrial Dysfunction, and cGAS-STING Activation |
