Human CXCL4/PF4 ELISA Kit
Cat no : KE00257
Synonyms
C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4
Validation Data Gallery
Product Information
KE00257 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human CXCL4/PF4 ELISA kit is to be used to detect and quantify protein levels of endogenous human CXCL4/PF4. The assay recognizes human CXCL4/PF4. An antibody specific for human CXCL4/PF4 has been pre-coated onto the microwells. The human CXCL4/PF4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL4/PF4 is added to detect the captured human CXCL4/PF4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CXCL4/PF4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.08 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5196 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88% | 74%-100% |
| Cell culture supernatants | 99% | 88%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 486.9 | 19.9 | 4.1 |
| 2 | 20 | 110.7 | 6.1 | 5.5 |
| 3 | 20 | 26.3 | 1.2 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 504.2 | 16.4 | 3.3 |
| 2 | 24 | 116.1 | 3.6 | 3.1 |
| 3 | 24 | 28.9 | 2.3 | 7.9 |
Background Information
CXCL4, alternatively known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Sleep Med Platelet-activating cytokines potentially associated with MASLD-induced liver injury significantly decreased following CPAP therapy: A translational study using a fatty liver mouse model | ||
Br J Haematol PF4 promotes CXCR3+ Tfh1 cell differentiation through STAT1 in mouse immune thrombocytopenia |
