Human CXCL16 ELISA Kit
Cat no : KE00052
Synonyms
C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16
Validation Data Gallery
Product Information
KE00052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL16 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL16. The assay recognizes human CXCL16. An antibody specific for CXCL16 has been pre-coated onto the microwells. The CXCL16 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL16 is added to detect the captured CXCL16 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CXCL16 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 58191 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 76%-103% |
| Human plasma | 99% | 83%-119% |
| Cell culture supernatants | 87% | 82%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 241.1 | 10.8 | 4.5 |
| 2 | 20 | 56.1 | 1.7 | 3.0 |
| 3 | 20 | 12.7 | 0.5 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 260.7 | 17.1 | 6.6 |
| 2 | 24 | 56.0 | 3.2 | 5.8 |
| 3 | 24 | 12.7 | 0.7 | 5.8 |
Background Information
CXC chemokine ligand 16 (CXCL16) is a CXC soluble chemokine, an adhesion molecule and a cell surface scavenger receptor. CXCL16 regulates inflammation, tissue injury and fibrosis. CXCL16 exists as a transmembrane form (TM-CXCL16) as well as a soluble form (sCXCL16) that is cleaved by proteolytic enzymes. CXCL16 is produced by several inflammatory cells including macrophages, dendritic cells, smooth muscle cells or lymphocytes. CXCL16 is expressed in various cancers, including pancreatic, prostate, breast, colorectal, and nonsmall cell lung cancer. CXCL16) has been proposed as an important pathogenic mediator in inflammatory diseases, such as rheumatoid arthritis, hepatitis or atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Am J Pathol Low-Dose Acetylsalicylic Acid Treatment Modulates the Production of Cytokines and Improves Trophoblast Function in an in Vitro Model of Early-Onset Preeclampsia. | ||
Exp Dermatol Reduced expression of CXCL16/CXCR6 is involved in the pathogenesis of hidradenitis suppurativa |
