Human CPD ELISA Kit
Validation Data Gallery
Product Information
KE00714 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CPD ELISA kit is to be used to detect and quantify protein levels of endogenous human CPD. The assay recognizes human CPD. An antibody specific for human CPD has been pre-coated onto the microwells. The human CPD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CPD is added to detect the captured human CPD protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CPD ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1362 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 80%-88% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 11.66 | 0.41 | 3.51 |
| 2 | 8 | 3.17 | 0.08 | 2.57 |
| 3 | 8 | 0.99 | 0.07 | 7.10 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.94 | 0.49 | 4.13 |
| 2 | 16 | 3.16 | 0.10 | 3.07 |
| 3 | 16 | 0.95 | 0.07 | 6.86 |
Background Information
CPD is a membrane-bound glycoprotein with three carboxypeptidase-like domains, but only the first two domains have enzymatic activity. It plays a key role in the processing and modification of protein, ensuring the correct folding and function of the newly synthesized protein. CPD is a membrane-bound component of Golgi negative network, which circulates to the cell surface through secretion and endocytosis. The results show that the two active domains of CPD have different preferences for substrates. The first domain prefers C-terminal arginine, while the second domain has the same number of substrates for lysine and arginine. Although the specific role of CPD in some diseases has not been fully understood, studies have shown that its abnormal expression may be related to the development of cancer, especially in tumor cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | carboxypeptidase D, GP180 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Photochem Photobiol Sci Inonotus obliquus polysaccharides alleviate UVB-irradiated skin photodamage by regulating calcium homeostasis through endoplasmic reticulum-mitochondria crosstalk. |
