Human CEACAM3 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00677
Synonyms
Carcinoembryonic antigen CGM1, Carcinoembryonic antigen-related cell adhesion molecule 3, CD66D, CGM1, W264
Validation Data Gallery
Product Information
KE00677 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CEACAM3 ELISA kit is to be used to detect and quantify protein levels of endogenous human CEACAM3. The assay recognizes human CEACAM3. An antibody specific for human CEACAM3 has been pre-coated onto the microwells. The human CEACAM3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CEACAM3 is added to detect the captured human CEACAM3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEACAM3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1084 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 123% | 116%-130% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.30 | 0.17 | 3.15 |
| 2 | 8 | 1.26 | 0.06 | 4.38 |
| 3 | 8 | 0.60 | 0.01 | 1.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.31 | 0.23 | 4.42 |
| 2 | 16 | 1.23 | 0.06 | 4.67 |
| 3 | 16 | 0.62 | 0.03 | 4.25 |
Background Information
CEA cell adhesion molecule 3 (CEACAM3) is an immunoglobulin-related glycoprotein that plays a crucial role in the innate immune response. It is expressed exclusively on granulocytes and is involved in the opsonin-independent recognition and elimination of specific Gram-negative bacterial pathogens. Unlike other CEACAM family members, CEACAM3 does not support cell-cell adhesion but functions as a receptor for pathogen recognition and elimination. Additionally, CEACAM3 has been suggested to have potential applications in cancer immunotherapy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Carcinoembryonic antigen CGM1, Carcinoembryonic antigen-related cell adhesion molecule 3, CD66D, CGM1, W264 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
