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Recombinant Human B7-2/CD86 protein (His Tag)

ED50

72-290 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

942

Accession

P42081

Cat No : Eg0966

Print datasheet

Synonyms

B70, B7-2, BU63, CD28LG2, CD86, LAB72, MGC34413

验证数据展示

  • Purity of Recombinant Human B7-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human B7-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human B7-2 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 72-290 ng/mL.

    Immobilized Human B7-2 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 72-290 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human B7-2 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 72-290 ng/mL.
Source HEK293-derived Human B7-2 protein Leu26-Pro247 (Accession# P42081) with His tag at the C-terminus.
Predicted Molecular Mass 26.2 kDa
SDS-PAGE 37-60 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • 12 months from the date of receipt, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin (Ig) superfamily. CD86 is primarily expressed in antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages.CD86 has strong structural similarity with another B7 family molecule, CD80(B7-1).CD86 and CD80 are the ligands for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte antigen 4 (CTLA-4). The binding of CD86 or  CD80 with CD28 antigen is a costimulatory signal for T cell activation, proliferation, and cytokine production. The binding of CD86 or CD80 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. However, CD86 and CD80 bind to CTLA-4 with higher affinity than CD28. Defects in CTLA-4-mediated transendocytosis of CD86 are associated with autoimmunity.

References:

1.Bolandi N, et al. (2021). Int J Mol Sci. 22(19):10719
2.Yokozeki H, et al. (1996). J Invest Dermatol.106(1):147-153
3.Baravalle G, et al. (2011). J Immunol. 187(6):2966-2973. 
4.Collins M, et al. (2005). Genome Biol. 6(6):223
5.Greaves P, et al. (2013). Blood. 121(5):734-744
6.Kennedy A, et al. (2022). Nat Immunol.23(9):1365-1378