Speedy™ Human CD6 One-Step ELISA Kit
Sensitivity
7.1 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : SE50089
Synonyms
CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6, TP120
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD6 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 923 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 120% | 111%-126% |
| Cell lysate | 80% | 76%-83% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,693.5 | 41.7 | 1.5 |
| 2 | 8 | 643.1 | 7.3 | 1.1 |
| 3 | 8 | 324.4 | 5.5 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,688.3 | 47.4 | 1.8 |
| 2 | 16 | 649.7 | 12.7 | 2.0 |
| 3 | 16 | 325.0 | 5.4 | 1.7 |
Background Information
CD6 is a lymphocyte surface accessory molecule that is associated with the T-cell receptor (TCR) complex in the immune synapse. It plays a key role in T-cell activation and proliferation by mediating cell-cell contacts and regulating T-cell responses. CD6 is involved in the stabilization and maturation of the immunological synapse, facilitating T-cell interactions with antigen-presenting cells (APCs) and subsequent T-cell proliferative responses. Additionally, CD6 has been shown to modulate T-cell activation by attenuating signaling cascades, acting as a rheostat-type signalosome that tunes T-cell activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6, TP120 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
