Recombinant Human CD47 protein (Myc Tag, His Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
Myc Tag, His Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <1.0 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human t-Plasminogen activator protein Gln19-Pro139 (Accession# Q08722-1, Tyr54Cys) with a Myc tag and a His tag at the C-terminus. |
| 基因ID | 961 |
| 蛋白编号 | Q08722-1 |
| 预测分子量 | 19.3 kDa |
| SDS-PAGE | |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
参考文献:
1. Brown, E. The Journal of clinical investigation vol. 107,12 (2001): 1499-500. 2. Sick, E et al. British journal of pharmacology vol. 167,7 (2012): 1415-30. 3. Barclay, A Neil, and Timo K Van den Berg. Annual review of immunology vol. 32 (2014): 25-50.