Speedy™ Human CD4 One-Step ELISA Kit
Sensitivity
4.9 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : SE50064
Synonyms
CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 920 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 96% | 91%-101% |
| Cell lysate | 89% | 84%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,126.8 | 37.2 | 3.3 |
| 2 | 8 | 273.1 | 2.9 | 1.1 |
| 3 | 8 | 139.4 | 3.4 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,154.8 | 66.1 | 5.7 |
| 2 | 16 | 273.9 | 2.9 | 1.1 |
| 3 | 16 | 140.2 | 4.0 | 2.9 |
Background Information
CD4 is a transmembrane glycoproteinof the immunoglobulin super-family (IgSF), containing four extracellular Ig-like domains, ahydrophobic transmembrane region and a 40-residue cytoplasmictail. CD4 is expressed T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells.CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interactionand it is involved in T cell differentiation, development and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 associates with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes.CD4 also serves as a receptor for human immunodeficiency virus (HIV) by binding to the viral envelope glycoprotein gp120.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
