Human CD3E ELISA Kit
Sensitivity
31.8 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00026 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD3E ELISA kit is to be used to detect and quantify protein levels of endogenous CD3E. The assay recognizes human CD3E. An antibody specific for CD3E has been pre-coated onto the microwells. The CD3E protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CD3E is added to detect the captured human CD3E protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD3E ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 31.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 916 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 105% | 73%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,083.4 | 163.6 | 7.9 |
| 2 | 20 | 454.2 | 31.2 | 6.9 |
| 3 | 20 | 119.1 | 6.9 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1.825.7 | 162.8 | 8.9 |
| 2 | 20 | 452.6 | 26.1 | 5.8 |
| 3 | 20 | 115.6 | 11.3 | 9.8 |
Background Information
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, and zeta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3E encodes the CD3-epsilon polypeptide which plays an essential role in T-cell development in addition to its role of signal transduction in T-cell activation. Defects in CD3E cause immunodeficiency.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD3E, T3E, TCRE |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
