Human CD27 ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00886
Synonyms
CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27
Validation Data Gallery
Product Information
KE00886 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD27 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD27. The assay recognizes human CD27. An antibody specific for human CD27 has been pre-coated onto the microwells. The human CD27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD27 is added to detect the captured human CD27 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD27 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 939 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 79%-111% |
| Cell culture supernatant | 99% | 84%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.19 | 0.06 | 1.08 |
| 2 | 8 | 1.25 | 0.05 | 3.81 |
| 3 | 8 | 0.39 | 0.01 | 3.72 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.36 | 0.21 | 4.00 |
| 2 | 16 | 1.30 | 0.06 | 4.68 |
| 3 | 16 | 0.40 | 0.02 | 4.86 |
Background Information
CD27 (also known as TNFRSF7) is a lymphocyte-specific member of the TNF/NGF-R family, which is found in NK, T, and B cell populations. CD27 is a T and B cell co-stimulatory molecule. Its activity is governed by the transient availability of its TNF-like ligand CD70 on lymphocytes and dendritic cells. This interaction may have a key role in controlling the size and function of antigen-primed lymphocyte populations. CD27 is also required for the effective generation and long-term maintenance of antigen-specific CD4+ and CD8+ T cell immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
