Human CD163 ELISA Kit
Validation Data Gallery
Product Information
KE00190 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD163 ELISA kit is to be used to detect and quantify protein levels of endogenous CD163. The CD163 ELISA kit is designed to measure human soluble CD163 in serum and plasma. The assay recognizes human CD163. An antibody specific for CD163 has been pre-coated onto the microwells. The CD163 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CD163 is added to detect the captured human CD163 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD163 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.2 ng/mL |
| Range | 6.25 - 400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9332 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 78%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 44.3 | 1.2 | 2.8 |
| 2 | 20 | 64.2 | 1.7 | 2.7 |
| 3 | 20 | 195.9 | 6.7 | 3.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 50.9 | 3.7 | 7.2 |
| 2 | 24 | 76.4 | 6.2 | 8.1 |
| 3 | 24 | 247.5 | 21.3 | 8.6 |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily. It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages. CD163 mediates endocytosis of haptoglobin-haemoglobin complexes. The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD163,CD163 molecule,Hemoglobin scavenger receptor |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Proteome Res Longitudinal Serum Proteomics Characterization of CD19-CAR-T Cell Therapy for B-Cell Malignancies | ||
