Human CD16 ELISA Kit
Sensitivity
0.13 ng/mL
Range
0.39-25 ng/mL
Reactivity
Human
Cat no : KE00284
Synonyms
CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1
Validation Data Gallery
Product Information
KE00284 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD16 ELISA kit is to be used to detect and quantify protein levels of endogenous CD16. The assay recognizes human CD16. An antibody specific for CD16 has been pre-coated onto the microwells. The CD16 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for CD16 is added to detect the captured CD16 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD16 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.13 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2215 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 96% | 71%-124% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 12.58 | 0.43 | 3.5 |
| 2 | 20 | 3.07 | 0.08 | 2.7 |
| 3 | 20 | 0.82 | 0.04 | 5.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 13.24 | 0.49 | 3.7 |
| 2 | 24 | 3.22 | 0.11 | 3.5 |
| 3 | 24 | 0.79 | 0.06 | 7.0 |
Background Information
CD16, also known as the low affinity Fc gamma receptor III for IgG (FcγRIII), exists in two isoforms, FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. CD16a is expressed on NK cells, macrophages, and placental trophoblasts as a polypeptide-anchored transmembrane protein while CD16b is expressed on neutrophils in a glycosylphosphatidylinositol (GPI)-anchored form. CD16a forms a heteromeric structure with the Fc epsilon RI (gamma) and/or CD3 (zeta) subunits. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 can be cleaved and released as a soluble form (sCD16).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
