Human CD147 ELISA Kit
Validation Data Gallery
Product Information
KE00135 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD147 ELISA kit is to be used to detect and quantify protein levels of endogenous CD147. The assay recognizes human CD147. An antibody specific for CD147 has been pre-coated onto the microwells. The CD147 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CD147 is added to detect the captured human CD147 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD147 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 682 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 101% | 75%-121% |
| Human serum | 97% | 76%-118% |
| Cell culture supernatants | 93% | 80%-115% |
| Human milk | 104% | 88%-125% |
| Human urine | 104% | 78%-121% |
| Human Saliva | 97% | 84%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 56.5 | 1.1 | 2.0 |
| 2 | 20 | 219.7 | 6.7 | 3.1 |
| 3 | 20 | 816.5 | 33.5 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 56.2 | 2.5 | 4.4 |
| 2 | 24 | 221.9 | 9.6 | 4.3 |
| 3 | 24 | 865.6 | 58.0 | 6.7 |
Background Information
CD147, also called Basigin (BSG) or EMMPRIN, is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily and is important in numerous physiological and pathological phenomena. It is involved in reproduction, neural function, inflammation and tumor invasion. CD147 is broadly distributed, and expressed at particularly high levels at the retinal pigment epithelium and neonatal blood-brain barrier, on tumour cells and on activated T cells. CD147 can stimulate adjacent fibroblasts to produce matrix metalloproteinases (MMPS). It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers. Soluble CD147 (sCD147) has been uncovered in addition to membrane-bound CD147. sCD147 production is mediated by two different mechanisms: microvesicular secretion and proteolytic cleavage. The presence of sCD147 in body fluids is associated with several diseases such as systemic sclerosis, hepatocellular carcinoma, ankylosing spondylitis and coronary diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Clin Pediatr (Phila) Association of High Plasma Levels of Serpin E1, IGFBP2, and CCL5 With Refractory Epilepsy in Children by Cytokine Profiling |
