Speedy™ Human CD134/OX40 One-Step ELISA Kit
Sensitivity
10.9 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD134/OX40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 10.9 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 92%-102% |
| Cell culture supernatant | 106% | 97%-114% |
| Cell lysate | 80% | 75%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,323.5 | 24.8 | 1.9 |
| 2 | 8 | 329.5 | 9.7 | 2.9 |
| 3 | 8 | 163.4 | 4.0 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,324.5 | 26.1 | 2.0 |
| 2 | 16 | 329.2 | 9.9 | 3.0 |
| 3 | 16 | 164.0 | 5.3 | 3.2 |
Background Information
OX40 (CD134) is a co-stimulatory receptor belonging to the TNF receptor family. It is expressed on activated CD4+ T cells and plays a crucial role in enhancing T cell proliferation, cytokine production, and survival. OX40 signaling leads to the activation of NF-κB and the upregulation of anti-apoptotic proteins such as Bcl-xL and Bcl-2. This receptor also promotes the differentiation of naive CD4+ T cells into high IL-4-producing effectors. OX40 ligand (OX40L) is expressed on antigen-presenting cells (APCs) such as activated B cells and dendritic cells. The interaction between OX40 and OX40L is critical for T cell activation and the regulation of immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF4, ACT35, ACT35 antigen, CD134, OX40 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
