Human OX40/CD134 ELISA Kit
Validation Data Gallery
Product Information
KE00209 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The OX40/CD134 ELISA kit is to be used to detect and quantify protein levels of endogenous OX40/CD134. The assay recognizes human OX40/CD134. An antibody specific for OX40/CD134 has been pre-coated onto the microwells. The OX40/CD134 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for OX40/CD134 is added to detect the captured OX40/CD134 protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human OX40/CD134 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 84%-110% |
| Human plasma | 107% | 98%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 79.1 | 2.8 | 3.6 |
| 2 | 20 | 290.5 | 9.1 | 3.1 |
| 3 | 20 | 1,270.6 | 67.0 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 68.9 | 5.8 | 8.4 |
| 2 | 24 | 278.3 | 25.4 | 9.1 |
| 3 | 24 | 1,144.6 | 59.9 | 5.2 |
Background Information
OX40 (CD134) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, OX40 is activated by its cognate ligand OX40L (CD134L) and functions as a T cell co-stimulatory molecule. OX40-OX40L interactions have been proposed as a potential therapeutic target for treating autoimmunity. OX40 can interact with TRAF2, TRAF3, and TRAF5. In addition to a membrane bound form, OX40 also exists as a soluble form, released by activated lymphocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACT35, ACT35 antigen, CD134, OX40, OX40L receptor, TNFRSF4, TXGP1L |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Arthritis Res Ther Identification of candidate biomarkers for diagnosis, disease activity, and relapse prediction in IgG4-related disease. |
