Human CCL7/MCP-3 ELISA Kit
Cat no : KE00262
Synonyms
C C motif chemokine 7, CCL7, chemokine (C C motif) ligand 7, FIC, MARC, MCP 3, MCP3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small inducible cytokine A7
Validation Data Gallery
Product Information
KE00262 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL7 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL7. The assay recognizes human CCL7. An antibody specific for CCL7 has been pre-coated onto the microwells. The CCL7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL7 is added to detect the captured human CCL7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL7/MCP-3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6354 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88% | 74%-100% |
| Cell culture supernatants | 105% | 94%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 13.3 | 0.5 | 3.4 |
| 2 | 20 | 57.1 | 1.0 | 1.8 |
| 3 | 20 | 218.7 | 4.9 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 13.8 | 1.0 | 7.3 |
| 2 | 24 | 55.3 | 1.0 | 1.8 |
| 3 | 24 | 210.0 | 7.6 | 3.6 |
Background Information
The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10. Various studies have shown that tumor cells and stromal cells also produce high levels of CCL7, while the specific response element and signaling pathways involved are not entirely clear.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C C motif chemokine 7, CCL7, chemokine (C C motif) ligand 7, FIC, MARC, MCP 3, MCP3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small inducible cytokine A7 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology Hypoxic glioma-derived exosomal miR-25-3p promotes macrophage M2 polarization by activating the PI3K-AKT-mTOR signaling pathway |
