Speedy™ Human CCL22/MDC One-Step ELISA Kit
Sensitivity
2.7 pg/mL
Range
25-1600 pg/mL
Reactivity
Human
Cat no : SE50033
Synonyms
CCL22, MDC, C C motif chemokine 22, CC chemokine STCP-1, C-C motif chemokine 22
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CCL22/MDC One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.7 pg/mL |
| Range | 25-1600 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6367 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 111% | 101%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 901.0 | 36.4 | 4.0 |
| 2 | 8 | 208.5 | 15.0 | 7.2 |
| 3 | 8 | 97.6 | 1.7 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 916.9 | 32.0 | 3.5 |
| 2 | 16 | 206.0 | 13.1 | 6.4 |
| 3 | 16 | 97.3 | 2.4 | 2.5 |
Background Information
The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL22, MDC, C C motif chemokine 22, CC chemokine STCP-1, C-C motif chemokine 22 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
