Recombinant Human C5AR1/CD88 protein (rFc Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg2185

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Synonyms



产品信息

纯度 >90 %, SDS-PAGE
内毒素 <1.0 EU/μg protein, LAL method
生物活性
Not tested
来源 HEK293-derived Human C5AR1/CD88 protein Met1-Asp37 (Accession# P21730) with a rabbit IgG Fc tag at the C-terminus.
基因ID 728
蛋白编号 P21730
预测分子量 30.5 kDa
SDS-PAGE
组分 Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

C5aR, also named as CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa.

参考文献:

1.Gerard, N P, and C Gerard. Nature vol. 349,6310 (1991): 614-7. 2. Schieferdecker, H L et al. FEBS letters vol. 406,3 (1997): 305-9. 3.Boulay, F et al. Biochemistry vol. 30,12 (1991): 2993-9.