Human ATG7 ELISA Kit
Cat no : KE00276
Synonyms
APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7
Validation Data Gallery
Product Information
KE00276 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ATG7 ELISA kit is to be used to detect and quantify protein levels of endogenous human ATG7. The assay recognizes human ATG7. An antibody specific for human ATG7 has been pre-coated onto the microwells. The human ATG7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ATG7 is added to detect the captured human ATG7 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ATG7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 25.5 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10533 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 91% | 73%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4,302.5 | 161.9 | 3.8 |
| 2 | 20 | 1,096.8 | 15.3 | 1.4 |
| 3 | 20 | 302.1 | 9.9 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4,338.3 | 188.0 | 4.3 |
| 2 | 24 | 1,106.0 | 38.4 | 3.5 |
| 3 | 24 | 282.1 | 18.9 | 6.7 |
Background Information
ATG7(autophagy-related gene 7), is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. ATG7 acts as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. ATG7 also regulates immunity, cell death and protein secretion, and independently regulates the cell cycle and apoptosis. ATG7 has been reported to be associated with growth and invasion through multiple mechanisms in a few cancers, such as hepatocellular carcinoma, bladder cancer. The high expression level of ATG7 is related to the survival of patients with breast cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Clin Transl Hepatol Hydrogen Sulfide Promotes Platelet Autophagy via PDGFR-α/PI3K/Akt Signaling in Cirrhotic Thrombocytopenia |
