Human APOA1 ELISA Kit
Validation Data Gallery
Product Information
KE00157 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOA1 ELISA kit is to be used to detect and quantify protein levels of endogenous APOA1. The assay recognizes human APOA1. An antibody specific for APOA1 has been pre-coated onto the microwells. The APOA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for APOA1 is added to detect the captured APOA1 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APOA1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 335 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 78%-122% |
| Cell culture supernatants | 105% | 87%-124% |
| Urine | 84% | 74%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 15.0 | 0.6 | 4.2 |
| 2 | 20 | 5.9 | 0.1 | 2.4 |
| 3 | 20 | 3.7 | 0.1 | 3.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 14.6 | 0.4 | 2.9 |
| 2 | 24 | 6.0 | 0.2 | 4.0 |
| 3 | 24 | 3.6 | 0.2 | 5.3 |
Background Information
APOA1 (apolipoprotein A1) is a plasma protein and a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of APOA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of APOA1 is inversely related to the risk of developing atherosclerosis. Alterations of APOA1 level also correlate to cancer and Alzheimer's Disease, thus measurement of APOA1 can be used in diagnosis or prognosis of related diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Alzheimers Dis Alpha-1-acid glycoprotein as a potential serum biomarker for cerebral amyloid angiopathy | ||
Obes Surg Relationship Between Weight Loss, Changes in Serum hs-CRP Levels and apo A-1 Lipoprotein, and High-Density Lipoprotein-Cholesterol Ratios as Predictors for Improved Cardiovascular Risk Factors After Laparoscopic Sleeve Gastrectomy |
