Human ANP32B ELISA Kit
Sensitivity
3.1 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00645
Synonyms
Acidic leucine-rich nuclear phosphoprotein 32 family member B, APRIL, PHAPI2, Putative HLA-DR-associated protein I-2, Silver-stainable protein SSP29
Validation Data Gallery
Product Information
KE00645 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ANP32B ELISA kit is to be used to detect and quantify protein levels of endogenous human ANP32B. The assay recognizes human ANP32B. An antibody specific for human ANP32B has been pre-coated onto the microwells. The human ANP32B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ANP32B is added to detect the captured human ANP32B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ANP32B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10541 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 111% | 100%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,058.3 | 30.1 | 2.8 |
| 2 | 8 | 230.4 | 12.8 | 5.6 |
| 3 | 8 | 125.1 | 4.5 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,113.6 | 75.7 | 6.8 |
| 2 | 16 | 230.0 | 9.3 | 4.0 |
| 3 | 16 | 124.7 | 3.8 | 3.0 |
Background Information
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Acidic leucine-rich nuclear phosphoprotein 32 family member B, APRIL, PHAPI2, Putative HLA-DR-associated protein I-2, Silver-stainable protein SSP29 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
