Human AKAP1 ELISA Kit
Sensitivity
0.5 pg/mL
Range
4.7-300 pg/mL
Reactivity
Human
Cat no : KE00908
Synonyms
AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, DAKAP1
Validation Data Gallery
Product Information
KE00908 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AKAP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human AKAP1. The assay recognizes human AKAP1. An antibody specific for human AKAP1 has been pre-coated onto the microwells. The human AKAP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AKAP1 is added to detect the captured human AKAP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AKAP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 4.7-300 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8165 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 93%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 188.3 | 7.1 | 3.8 |
| 2 | 8 | 41.0 | 2.4 | 5.9 |
| 3 | 8 | 23.1 | 1.3 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 193.4 | 9.9 | 5.1 |
| 2 | 16 | 41.7 | 2.5 | 6.1 |
| 3 | 16 | 22.1 | 1.7 | 7.6 |
Background Information
AKAP1 (A-Kinase Anchoring Protein 1), also known as D-AKAP1 or AKAP149, is a mitochondrial and endoplasmic reticulum (ER)-anchored scaffolding protein that plays a critical role in organizing signaling complexes, particularly those involving protein kinase A (PKA). By tethering PKA and other signaling molecules to specific subcellular compartments, AKAP1 regulates essential cellular processes such as mitochondrial dynamics, apoptosis, metabolism, and oxidative stress response. AKAP1 contains a dual-specificity domain that binds both type I and type II regulatory subunits of PKA, along with additional binding sites for phosphatases, phosphodiesterases, and other kinases. Its localization at the outer mitochondrial membrane (OMM) allows it to modulate mitochondrial function, including fission/fusion balance and reactive oxygen species (ROS) management. Studies have linked AKAP1 to cardiovascular diseases, neurodegenerative disorders, and cancer, highlighting its importance in cellular homeostasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, DAKAP1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
