Human ADAMTS13 ELISA Kit

Sensitivity

0.0028 ng/mL

Range

0.625-40 ng/mL

Reactivity

Human

Cat No : KE00286

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Synonyms

ADAM TS 13, ADAM TS13, ADAMTS 13, ADAMTS13, C9orf8, DKFZp434C2322, FLJ42993, TTP, vWF cleaving protease, vWF CP, VWFCP



Product Information

KE00286 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ADAMTS13 ELISA kit is to be used to detect and quantify protein levels of endogenous human ADAMTS13. The assay recognizes human ADAMTS13. An antibody specific for human ADAMTS13 has been pre-coated onto the microwells. The human ADAMTS13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ADAMTS13 is added to detect the captured human ADAMTS13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human ADAMTS13 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant
Assay type Sandwich
Sensitivity 0.0028 ng/mL
Range 0.625-40 ng/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 11093

Recovery

Sample TypeAverageRange
Human serum98%84%-120%
Cell culture supernatant89%71%-109%

IntraAssay

Samplenmean ( ng/mL)SDCV%
12020.761.055.04
2204.960.255.07
3201.140.065.01

InterAssay

Samplenmean ( ng/mL)SDCV%
12423.541.988.39
2245.500.295.23
3241.290.086.52

Background Information

A disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) is a multidomain metalloprotease for which until now only a single substrate has been identified. ADAMTS13 cleaves the polymeric force-sensor von Willebrand factor (VWF) that unfolds under shear stress and recruits platelets to sites of vascular injury. In patients with immune-mediated thrombotic thrombocytopenic purpura (iTTP), a rare life threatening disease, ADAMTS13 is targeted by autoantibodies that inhibit its activity or promote its clearance. In the absence of ADAMTS13, VWF polymers are not adequately processed, resulting in spontaneous adhesion of blood platelets, which presents as severe, life-threatening microvascular thrombosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms ADAM TS 13, ADAM TS13, ADAMTS 13, ADAMTS13, C9orf8, DKFZp434C2322, FLJ42993, TTP, vWF cleaving protease, vWF CP, VWFCP