Human ADAMTS13 ELISA Kit
Cat no : KE00286
Synonyms
ADAM TS 13, ADAM TS13, ADAMTS 13, ADAMTS13, C9orf8, DKFZp434C2322, FLJ42993, TTP, vWF cleaving protease, vWF CP, VWFCP
Validation Data Gallery
Product Information
KE00286 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ADAMTS13 ELISA kit is to be used to detect and quantify protein levels of endogenous human ADAMTS13. The assay recognizes human ADAMTS13. An antibody specific for human ADAMTS13 has been pre-coated onto the microwells. The human ADAMTS13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ADAMTS13 is added to detect the captured human ADAMTS13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ADAMTS13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.0028 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11093 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 84%-120% |
| Cell culture supernatant | 89% | 71%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 20.76 | 1.05 | 5.04 |
| 2 | 20 | 4.96 | 0.25 | 5.07 |
| 3 | 20 | 1.14 | 0.06 | 5.01 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 23.54 | 1.98 | 8.39 |
| 2 | 24 | 5.50 | 0.29 | 5.23 |
| 3 | 24 | 1.29 | 0.08 | 6.52 |
Background Information
A disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) is a multidomain metalloprotease for which until now only a single substrate has been identified. ADAMTS13 cleaves the polymeric force-sensor von Willebrand factor (VWF) that unfolds under shear stress and recruits platelets to sites of vascular injury. In patients with immune-mediated thrombotic thrombocytopenic purpura (iTTP), a rare life threatening disease, ADAMTS13 is targeted by autoantibodies that inhibit its activity or promote its clearance. In the absence of ADAMTS13, VWF polymers are not adequately processed, resulting in spontaneous adhesion of blood platelets, which presents as severe, life-threatening microvascular thrombosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADAM TS 13, ADAM TS13, ADAMTS 13, ADAMTS13, C9orf8, DKFZp434C2322, FLJ42993, TTP, vWF cleaving protease, vWF CP, VWFCP |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Blood Vessel Thromb Hemost Elevated VWF with normal ADAMTS13 in pediatric sickle cell disease: links to crises and ischemic stroke. |
