Human ABCD1 ELISA Kit
Sensitivity
6.0 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00030 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ABCD1 ELISA kit is to be used to detect and quantify protein levels of endogenous ABCD1. The assay recognizes human ABCD1. An antibody specific for ABCD1 has been pre-coated onto the microwells. The ABCD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ABCD1 is added to detect the captured ABCD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ABCD1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 6.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 215 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 99% | 79%-123% |
| Human plasma | 93% | 85%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,689.5 | 138.9 | 8.2 |
| 2 | 20 | 1,243.4 | 117.6 | 9.5 |
| 3 | 20 | 104.7 | 3.6 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,599.1 | 122.2 | 7.6 |
| 2 | 24 | 836.0 | 50.7 | 6.1 |
| 3 | 24 | 105.2 | 4.2 | 4.0 |
Background Information
ABCD1, also known as adrenoleukodystrophy protein (ALDP), is a member of the ATP-binding cassette (ABC) transporter superfamily. ALDP is a multi-pass membrane protein and localized at peroxisome membrane. Various mutations of ABCD1 gene cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex. It results in mental deterioration, corticospinal tract dysfunction, and cortical blindness. ALDP is supposed to function as a homodimer allowing the entry of CoA-esters of very-long chain fatty acids into the peroxisome, the unique site of their β-oxidation. However, the defined function of ALDP has not been well investigated so far.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
