• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis using 66442-1-Ig (same clone as 66442-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66442-1-Ig (Hsc70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    Western blot analysis of Hsc70 in various cell lines using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    IF Staining of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Hsc70 antibody (66442-1-Ig, Clone: 1A4B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • WB analysis of Recombinant protein using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis of Recombinant protein using 66442-1-Ig (same clone as 66442-1-PBS)

    6*His tagged-recombinant HSP70 (291-641 aa) and HSC70 (346-646 aa) proteins were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody), 66442-1-Ig (HSC70 antibody), and 66005-1-Ig (His antibody), respectively, at dilution of 1:50000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    WB analysis of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    WB result of Hsc70 antibody (66442-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Hsc70 transfected HEK-293 cells. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSC70/HSPA8 antibody included
  • IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSC70/HSPA8 antibody included
  • IF Staining of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    IF Staining of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Hsc70 antibody (66442-1-Ig, Clone: 1A4B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Hsc70 Monoclonal antibody, PBS Only

Hsc70 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66442-1-PBS

产品说明书

CloneNo. 1A4B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HSPA8, 1A4B3, EC:3.6.4.10, Heat shock cognate 71 kDa protein, Heat shock protein family A member 8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HSC70/HSPA8 Ready-To-Use IHC Kit. HSC70/HSPA8 primary antibody included.

产品信息

66442-1-PBS targets Hsc70 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17910

Product name: Recombinant human Hsc70 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 288-587 aa of BC007276

Sequence: QKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 8
别名 HSPA8, 1A4B3, EC:3.6.4.10, Heat shock cognate 71 kDa protein, Heat shock protein family A member 8
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007276
基因名称 HSC70
Gene ID (NCBI) 3312
RRIDAB_2881812
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP11142
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)