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验证数据展示

  • WB analysis using 68345-1-Ig (same clone as 68345-1-PBS)

    WB analysis using 68345-1-Ig (same clone as 68345-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68345-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • WB analysis using 68345-1-Ig (same clone as 68345-1-PBS)

    WB analysis using 68345-1-Ig (same clone as 68345-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68345-1-Ig (Histone H3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    IP experiment of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    IP result of anti-Histone H3 (IP:68345-1-Ig, 4ug; Detection:68345-1-Ig 1:2000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68345-1-Ig (same clone as 68345-1-PBS)

    IHC staining of human colon cancer using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68345-1-Ig (Histone H3 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse testis using 68345-1-Ig (same clone as 68345-1-PBS)

    IHC staining of mouse testis using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68345-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of A431 using 68345-1-Ig (same clone as 68345-1-PBS)

    IF Staining of A431 using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68345-1-Ig (same clone as 68345-1-PBS)

    IF Staining of MCF-7 using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68345-1-Ig (same clone as 68345-1-PBS)

    IF Staining of MCF-7 using 68345-1-Ig (same clone as 68345-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, labeled with CoraLite647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L),SA00014-10, magenta) at dilution of 1:400 and CoraLite Plus 488-conjugated ZO-1 antibody (CL488-21773, green), CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9, labeled with FlexAble CoraLite Plus 555 Antibody Labeling Kit for Mouse IgG2a, KFA042, orange). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68345-1-Ig (same clone as 68345-1-PBS)

    FC experiment of HepG2 using 68345-1-Ig (same clone as 68345-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Histone H3 (68345-1-Ig, Clone:1A2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50178-1

    Cytometric bead array in cell lysate using MP50178-1

    Cytometric bead array in cell lysate using MP50178-1, Phospho-Histone H3 (Ser10) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68345-1-PBS. Detection antibody: 66863-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

Histone H3 Monoclonal antibody, PBS Only (Capture)

Histone H3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Cat No. 68345-1-PBS

产品说明书

CloneNo. 1A2A3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, dog, wheat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

别名

HIST1H3A, 1A2A3, Histone H 3, HistoneH3, Histone-H3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

68345-1-PBS targets Histone H3 as part of a matched antibody pair:

MP50178-1: 68345-1-PBS capture and 66863-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, hamster, dog, wheat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, 1A2A3, Histone H 3, HistoneH3, Histone-H3
观测分子量15-17 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.