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验证数据展示

  • WB analysis using 13754-1-AP (same clone as 13754-1-PBS)

    WB analysis using 13754-1-AP (same clone as 13754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 13754-1-AP (same clone as 13754-1-PBS)

    IHC staining of mouse testis using 13754-1-AP (same clone as 13754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13754-1-AP (Histone H3.3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 13754-1-AP (same clone as 13754-1-PBS)

    IF Staining of A431 using 13754-1-AP (same clone as 13754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3.3 antibody (13754-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13754-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Histone H3.3 Polyclonal antibody, PBS Only

Histone H3.3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13754-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

H3F3A, HistoneH3.3, Histone H3, H3-3B, H3-3A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13754-1-PBS targets Histone H3.3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4696

Product name: Recombinant human Histone H3.3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-136 aa of BC038989

Sequence: MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 H3 histone, family 3A
别名 H3F3A, HistoneH3.3, Histone H3, H3-3B, H3-3A
计算分子量 136 aa, 15 kDa
观测分子量 15-17 kDa
GenBank蛋白编号BC038989
基因名称 Histone H3
Gene ID (NCBI) 3020
RRIDAB_2115140
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP84243
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].

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