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验证数据展示

  • WB analysis using 33612-1-AP (same clone as 33612-1-PBS)

    WB analysis using 33612-1-AP (same clone as 33612-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33612-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33612-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 33612-1-AP (same clone as 33612-1-PBS)

    IHC staining of mouse brain using 33612-1-AP (same clone as 33612-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33612-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33612-1-PBS in a different storage buffer formulation.

Histone H2B Polyclonal antibody, PBS Only

Histone H2B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 33612-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

H2B, H2B/a, H2B/g, H2B/h, H2B/k

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33612-1-PBS targets Histone H2B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag38954

Product name: Recombinant human HIST1H2BD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-126 aa of NM_021063.3

Sequence: MPEPTKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H2bd
别名 H2B, H2B/a, H2B/g, H2B/h, H2B/k
计算分子量14kDa，126aa
观测分子量16 kDa
GenBank蛋白编号NM_021063.3
基因名称 HIST1H2BD
Gene ID (NCBI) 3017
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP58876
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.

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