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验证数据展示

  • WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

    WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • IF Staining using 15857-1-AP (same clone as 15857-1-PBS)

    IF Staining using 15857-1-AP (same clone as 15857-1-PBS)

    Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

    IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

    IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15857-1-AP (same clone as 15857-1-PBS)

    IP experiment of HEK-293 using 15857-1-AP (same clone as 15857-1-PBS)

    IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

Histone H2B Polyclonal antibody, PBS Only

Histone H2B Polyclonal Antibody for WB, IHC, IF, IP, Indirect ELISA
Cat No. 15857-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF, IP, Indirect ELISA

别名

HIST2H2BE, GL105, H2B, H2B.1, H2B/q

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15857-1-PBS targets Histone H2B in WB, IHC, IF, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7811

Product name: Recombinant human Histone H2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-126 aa of BC005827

Sequence: MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 2, H2be
别名 HIST2H2BE, GL105, H2B, H2B.1, H2B/q
计算分子量 14 kDa
观测分子量14-17 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_10664929
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16778
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.

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