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验证数据展示

  • WB analysis using 18201-1-AP (same clone as 18201-1-PBS)

    WB analysis using 18201-1-AP (same clone as 18201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18201-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 18201-1-AP (same clone as 18201-1-PBS)

    IP experiment of mouse kidney using 18201-1-AP (same clone as 18201-1-PBS)

    IP result of anti-Histone H1 (IP:18201-1-AP, 4ug; Detection:18201-1-AP 1:500) with mouse kidney tissue lysate 4400ug. This data was developed using the same antibody clone with 18201-1-PBS in a different storage buffer formulation.

  • WB analysis using 18201-1-AP (same clone as 18201-1-PBS)

    WB analysis using 18201-1-AP (same clone as 18201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18201-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 18201-1-AP (same clone as 18201-1-PBS)

    IHC staining of human stomach using 18201-1-AP (same clone as 18201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18201-1-AP (Histone H1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18201-1-PBS in a different storage buffer formulation.

Histone H1 Polyclonal antibody, PBS Only

Histone H1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18201-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

H1, HIST1H1A, H1.1, H1-1, H1F1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18201-1-PBS targets Histone H1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12704

Product name: Recombinant human Histone H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-215 aa of BC101593

Sequence: MSETVPPAPAASAAPEKPLAGKKAKKPAKAAAASKKKPAGPSVSELIVQAASSSKERGGVSLAALKKALAAAGYDVEKNNSRIKLGIKSLVSKGTLVQTKGTGASGSFKLNKKASSVETKPGASKVATKTKATGASKKLKKATGASKKSVKTPKKAKKPAATRKSSKNPKKPKTVKPKKVAKSPAKAKAVKPKAAKARVTKPKTAKPKKAAPKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H1a
别名 H1, HIST1H1A, H1.1, H1-1, H1F1
计算分子量 215 aa, 22 kDa
观测分子量 28 kDa, 30 kDa
GenBank蛋白编号BC101593
基因名称 Histone H1
Gene ID (NCBI) 3024
RRIDAB_10859820
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02539
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone H1 is a key chromatin structural protein, which mediates higher-order chromatin folding. H1 is also emerging as an important epigenetic mark and regulator of gene expression and cellular differentiation.

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