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验证数据展示

  • WB analysis of NIH/3T3 using 82989-4-RR (same clone as 82989-4-PBS)

    WB analysis of NIH/3T3 using 82989-4-RR (same clone as 82989-4-PBS)

    Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82989-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 82989-4-RR (same clone as 82989-4-PBS)

    IHC staining of mouse kidney using 82989-4-RR (same clone as 82989-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-4-RR (Hif-1 alpha antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82989-4-RR

    Affinity and Kinetic Characterization of 82989-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.

Mouse Hif-1 alpha Recombinant monoclonal antibody, PBS Only

Hif-1 alpha Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82989-4-PBS

产品说明书

CloneNo. 230101E8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

Hif1a, 230101E8, ARNT-interacting protein, Hif-1a, HIF1alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82989-4-PBS targets Hif-1 alpha in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag33620

Product name: Recombinant mouse Hif1a protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 530-825 aa of NM_001313919

Sequence: SEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQG*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit
别名 Hif1a, 230101E8, ARNT-interacting protein, Hif-1a, HIF1alpha
计算分子量93 kDa
观测分子量120 kDa
GenBank蛋白编号NM_001313919
基因名称 Hif1a
Gene ID (NCBI) 15251
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ61221
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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