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验证数据展示

  • WB analysis of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    WB analysis of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 84128-5-RR (Her2 / ErbB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84128-5-PBS in a different storage buffer formulation.

  • IF Staining of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    IF Staining of 4T1 using 84128-5-RR (same clone as 84128-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using Her2 / ErbB2 antibody (84128-5-RR, Clone: 241366F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84128-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84128-5-RR

    Affinity and Kinetic Characterization of 84128-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84128-5-RR against Mouse Her2 / ErbB2 were performed. The affinity constant is 0.356 nM.

Mouse HER2/ErbB2 Recombinant antibody, PBS Only

HER2/ErbB2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84128-5-PBS

产品说明书

CloneNo. 241366F4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84128-5-PBS targets HER2/ErbB2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
别名 Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340
计算分子量139 kDa
观测分子量185 kDa
GenBank蛋白编号NM_001003817.1
基因名称 Erbb2
Gene ID (NCBI) 13866
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP70424
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.