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验证数据展示

  • WB analysis of human plasma using 86395-3-RR (same clone as 86395-3-PBS)

    WB analysis of human plasma using 86395-3-RR (same clone as 86395-3-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86395-3-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 86395-3-RR (same clone as 86395-3-PBS)

    WB analysis of human placenta using 86395-3-RR (same clone as 86395-3-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 86395-3-RR (HPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86395-3-PBS in a different storage buffer formulation.

  • FC experiment of Transfected CHO using 86395-3-RR (same clone as 86395-3-PBS)

    FC experiment of Transfected CHO using 86395-3-RR (same clone as 86395-3-PBS)

    1x10^6 CHO cells or Transfected CHO cells were intracellularly stained with 0.25 ug Hemopexin Recombinant monoclonal antibody (86395-3-RR, Clone:250345E5) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 86395-3-PBS in a different storage buffer formulation.

Hemopexin Recombinant monoclonal antibody, PBS Only

Hemopexin Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 86395-3-PBS

产品说明书

CloneNo. 250345E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

HPX, Beta 1B glycoprotein, Beta-1B-glycoprotein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.

产品信息

86395-3-PBS targets Hemopexin in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3082

Product name: Recombinant Human Hemopexin/HPX protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-462 aa of NM_000613.2

Sequence: TPLPPTSAHGNVAEGETKPDPDVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSTHHGPEYMRCSPHLVLSALTSDNHGATYAFSGTHYWRLDTSRDGWHSWPIAHQWPQGPSAVDAAFSWEEKLYLVQGTQVYVFLTKGGYTLVSGYPKRLEKEVGTPHGIILDSVDAAFICPGSSRLHIMAGRRLWWLDLKSGAQATWTELPWPHEKVDGALCMEKSLGPNSCSANGPGLYLIHGPNLYCYSDVEKLNAAKALPQPQNVTSLLGCTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hemopexin
别名 HPX, Beta 1B glycoprotein, Beta-1B-glycoprotein
计算分子量52 kDa
观测分子量 65-75 kDa
GenBank蛋白编号NM_000613.2
基因名称 Hemopexin
Gene ID (NCBI) 3263
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02790
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

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