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验证数据展示

  • WB analysis using 83702-5-RR

    WB analysis using 83702-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83702-5-RR (Haptoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83702-5-RR

    IHC staining of mouse liver using 83702-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83702-5-RR (Haptoglobin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83702-5-RR

    Affinity and Kinetic Characterization of 83702-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83702-5-RR against Mouse Haptoglobin were performed. The affinity constant is 0.291 nM.

Haptoglobin Recombinant antibody

Haptoglobin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83702-5-RR

产品说明书

CloneNo. 240785A1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Hp, HP 1, 240785A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse serum, mouse lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83702-5-RR targets Haptoglobin in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 haptoglobin
别名 Hp, HP 1, 240785A1
计算分子量39kDa
观测分子量 42 kDa
GenBank蛋白编号NM_017370.2
基因名称 Hp
Gene ID (NCBI) 15439
RRIDAB_3671304
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ61646
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473).

实验方案

Product Specific Protocols
WB protocol for Haptoglobin antibody 83702-5-RRDownload protocol
IHC protocol for Haptoglobin antibody 83702-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols