验证数据展示
经过测试的应用
| Positive IHC detected in | human testis tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24741-1-AP targets HYDIN in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | HYDIN fusion protein Ag10732 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydrocephalus inducing homolog (mouse) |
| 别名 | HYDIN, HYDIN1, HYDIN2, KIAA1864 |
| 计算分子量 | 721aa,82 kDa; 5120aa,560 kDa |
| GenBank蛋白编号 | BC028351 |
| 基因名称 | HYDIN |
| Gene ID (NCBI) | 54768 |
| RRID | AB_2879700 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q4G0P3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HYDIN (hydrocephalus inducing homolog), also named as HYDIN1 or HYDIN2, is a 5121 amino acid protein, which localizes in the cell projection HYDIN exists as seven alternatively spliced isoforms and is expressed in the ciliated ependymal cell layer of heart ventricle. HYDIN is also found in ciliated epithelial cells of developing spermatocytes, oviduct and bronchi and is required for ciliary motility. HYDIN gene mutation is involved in Ciliary dyskinesia primary 5.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HYDIN antibody 24741-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
