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  • Featured Product
  • KD/KO Validated

HYAL2 Polyclonal antibody

HYAL2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 51148-1-AP

Print datasheet

Synonyms

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

验证数据展示

  • WB analysis of mouse heart using 51148-1-AP

    WB analysis of mouse heart using 51148-1-AP

    mouse heart tissue and mouse liver tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 51148-1-AP

    IHC staining of human lung cancer using 51148-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 51148-1-AP

    IHC staining of human lung cancer using 51148-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 51148-1-AP

    IF Staining of HeLa using 51148-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51148-1-AP (HYAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, human lung tissue, mouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51148-1-AP targets HYAL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HYAL2 fusion protein Ag0582 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hyaluronoglucosaminidase 2
Synonyms Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000692
Gene Symbol HYAL2
Gene ID (NCBI) 8692
RRIDAB_2123391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HYAL2 antibody 51148-1-APDownload protocol
IHC protocol for HYAL2 antibody 51148-1-APDownload protocol
IF protocol for HYAL2 antibody 51148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Dev Cell

High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.

Authors - Takuya Ooki
  • KD Validated
View Article