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HYAL2 Polyclonal antibody

HYAL2 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 51148-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF, IHC, ELISA

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, human lung tissue, mouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51148-1-AP targets HYAL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 HYAL2 fusion protein Ag0582 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hyaluronoglucosaminidase 2
别名 Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2
计算分子量 54 kDa
观测分子量 60 kDa
GenBank蛋白编号BC000692
基因名称 HYAL2
Gene ID (NCBI) 8692
RRIDAB_2123391
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

实验方案

Product Specific Protocols
WB protocol for HYAL2 antibody 51148-1-APDownload protocol
IHC protocol for HYAL2 antibody 51148-1-APDownload protocol
IF protocol for HYAL2 antibody 51148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Dev Cell

High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.

Authors - Takuya Ooki
  • KD Validated
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