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HYAL2 Polyclonal antibody

HYAL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15115-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

hyaluronoglucosaminidase 2, Hyaluronidase-2, Hyaluronidase 2, Hyal-2, Hyal 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, Raji cells, mouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15115-1-AP targets HYAL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 HYAL2 fusion protein Ag7157 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hyaluronoglucosaminidase 2
别名 hyaluronoglucosaminidase 2, Hyaluronidase-2, Hyaluronidase 2, Hyal-2, Hyal 2
计算分子量 54 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC000692
基因名称 HYAL2
Gene ID (NCBI) 8692
RRIDAB_2123393
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

实验方案

Product Specific Protocols
WB protocol for HYAL2 antibody 15115-1-APDownload protocol
IHC protocol for HYAL2 antibody 15115-1-APDownload protocol
IF protocol for HYAL2 antibody 15115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Genet Med

Elucidating the clinical spectrum and molecular basis of HYAL2 deficiency.

Authors - James Fasham
  • KO Validated
humanIHC,IF,WB

Front Immunol

Complement protein C1q stimulates hyaluronic acid degradation via gC1qR/HABP1/p32 in malignant pleural mesothelioma

Authors - Andrea Balduit
MOUSEWB

PLoS Genet

PMID: 28081210

Authors - Martina M A Muggenthaler
  • KO Validated
humanIHC

Cancers (Basel)

Impact of Hyaluronic Acid on the Cutaneous T-Cell Lymphoma Microenvironment: A Novel Anti-Tumor Mechanism of Bexarotene

Authors - Tetsuya Ikawa
mouseWB

Dermatol Ther

Role of Momordica charantia in preventing the natural aging process of skin and sexual organs in mice.

Authors - Keiichi Hiramoto
mouseWB,IHC

Matrix Biol

Hyaluronan degradation by HYAL2 is essential for odontoblastic differentiation and migration of mouse dental papilla cells

Authors - Haiyan Huang
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