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验证数据展示

  • WB analysis of Raji using 66449-1-Ig (same clone as 66449-1-PBS)

    WB analysis of Raji using 66449-1-Ig (same clone as 66449-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51480-1

    Cytometric bead array sample test of MP51480-1

    Cytometric bead array sample test of MP51480-1, HVCN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66449-1-PBS. Detection antibody: 66449-2-PBS.

  • IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51480-1

    Cytometric bead array standard curve of MP51480-1

    Cytometric bead array standard curve of MP51480-1, HVCN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66449-1-PBS. Detection antibody: 66449-2-PBS. Standard:Ag5350. Range: 0.781-100 ng/mL.

HVCN1 Monoclonal antibody, PBS Only (Capture)

HVCN1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66449-1-PBS

产品说明书

CloneNo. 1E4C4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

1E4C4, HV1, Hydrogen voltage-gated channel 1, UNQ578/PRO1140, Voltage sensor domain-only protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66449-1-PBS targets HVCN1 as part of a matched antibody pair:

MP51480-1: 66449-1-PBS capture and 66449-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5350

Product name: Recombinant human HVCN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-103 aa of BC032672

Sequence: MATWDEKAVTRRAKVAPAERMSKFLRHFTVVGDDYHAWNINYKKWENEEEEEEEEQPPPTPVSGEEGRAAAPDVAPAPGPAPRAPLDFRGMLRKLFSSHRFQV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hydrogen voltage-gated channel 1
别名 1E4C4, HV1, Hydrogen voltage-gated channel 1, UNQ578/PRO1140, Voltage sensor domain-only protein
计算分子量 273 aa, 32 kDa
观测分子量 28-35 kDa, 40 kDa, 60 kDa
GenBank蛋白编号BC032672
基因名称 HVCN1
Gene ID (NCBI) 84329
RRIDAB_2881818
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96D96
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.

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