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验证数据展示

  • WB analysis using 12038-1-AP (same clone as 12038-1-PBS)

    WB analysis using 12038-1-AP (same clone as 12038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12038-1-AP (HURP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12038-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 12038-1-AP (same clone as 12038-1-PBS)

    IF Staining of HEK-293 using 12038-1-AP (same clone as 12038-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 12038-1-AP (HURP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12038-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HURP Polyclonal antibody, PBS Only

HURP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 12038-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

DAP-5, Discs large homolog 7, Disks large-associated protein 5, Disks large-associated protein DLG7, DLG7

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12038-1-PBS targets HURP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2667

Product name: Recombinant human HURP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 498-847 aa of BC010658

Sequence: IKETTCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIASKPKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVKLFSGLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLFLSIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTNKKEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILEEGETKISQSELFDNKSLTTECHLLDSPGLNCSNPFTQLERRHQEHARHISFGGNLITFSPLQPGEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 discs, large (Drosophila) homolog-associated protein 5
别名 DAP-5, Discs large homolog 7, Disks large-associated protein 5, Disks large-associated protein DLG7, DLG7
计算分子量 846 aa, 95 kDa
观测分子量120 kDa
GenBank蛋白编号BC010658
基因名称 HURP
Gene ID (NCBI) 9787
RRIDAB_10665363
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15398
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLGAP5, also named as DAP 5 or DLG7, is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Expression of DGL7 is found in cancer and stem cells. DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation (PMID: 24324629).

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