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验证数据展示

  • IF Staining of HepG2 using 83492-6-RR (same clone as 83492-6-PBS)

    IF Staining of HepG2 using 83492-6-RR (same clone as 83492-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-6-RR, Clone: 240496E12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83492-6-RR (same clone as 83492-6-PBS)

    FC experiment of HeLa using 83492-6-RR (same clone as 83492-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-6-RR, Clone:240496E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83492-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83492-6-RR

    Affinity and Kinetic Characterization of 83492-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83492-6-RR against Human HTRA2 were performed. The affinity constant is 9.83 nM.

HTRA2 Recombinant monoclonal antibody, PBS Only

HTRA2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83492-6-PBS

产品说明书

CloneNo. 240496E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

240496E12, EC:3.4.21.108, HtrA serine peptidase 2, OMI, Omi stress-regulated endoprotease

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83492-6-PBS targets HTRA2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8455

Product name: Recombinant human HTRA2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 133-458 aa of BC000096

Sequence: AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HtrA serine peptidase 2
别名 240496E12, EC:3.4.21.108, HtrA serine peptidase 2, OMI, Omi stress-regulated endoprotease
计算分子量 49 kDa
GenBank蛋白编号BC000096
基因名称 HTRA2
Gene ID (NCBI) 27429
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO43464
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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