验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, Caco-2 cells |
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21165-1-AP targets HTR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag15226 Product name: Recombinant human HTR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 301-388 aa of BC074755 Sequence: GYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTINGSTHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5-hydroxytryptamine (serotonin) receptor 4 |
| 别名 | 5 HT 4, 5 HT4, 5 HT4R, 5 hydroxytryptamine receptor 4, 5-HT4 |
| 计算分子量 | 388 aa, 44 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC074755 |
| 基因名称 | HTR4 |
| Gene ID (NCBI) | 3360 |
| RRID | AB_11182374 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13639 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HTR4 (5-hydroxytryptamine receptor 4, 5-HT4) is a G protein-coupled receptor (GPCR) belonging to the serotonin receptor family. Activated by the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT), HTR4 primarily couples to Gs proteins, leading to stimulation of adenylyl cyclase, increased intracellular cAMP levels, and activation of downstream signaling pathways. HTR4 is widely expressed in the gastrointestinal tract, central nervous system, and cardiovascular system, where it regulates gastrointestinal motility, neurotransmission, learning and memory, and cardiac function. In the nervous system, HTR4 has been implicated in cognitive processes and neuroprotection, while in the gut it promotes intestinal peristalsis and secretion.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HTR4 antibody 21165-1-AP | Download protocol |
| IHC protocol for HTR4 antibody 21165-1-AP | Download protocol |
| WB protocol for HTR4 antibody 21165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Study on the effects of the atractylodes macrocephala koidz-raphanus sativus l herb pair in alleviating senile constipation via the gut microbiota-SCFAs-5-HT axis | ||
Biomed Pharmacother Enteromorpha and polysaccharides from enteromorpha ameliorate loperamide-induced constipation in mice. | ||
Nutrients Living, Heat-Killed Limosilactobacillus mucosae and Its Cell-Free Supernatant Differentially Regulate Colonic Serotonin Receptors and Immune Response in Experimental Colitis | ||
J Dairy Sci Synbiotic yogurt containing konjac mannan oligosaccharides and Bifidobacterium animalis ssp. lactis BB12 alleviates constipation in mice by modulating the stem cell factor (SCF)/c-Kit pathway and gut microbiota. | ||
Pharm Biol Prokinetic effects of Citrus reticulata and Citrus aurantium extract with/without Bupleurum chinense using multistress-induced delayed gastric emptying models |
