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HTR2B Polyclonal antibody

HTR2B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26408-1-AP

Print datasheet

Synonyms

5 HT 2B, 5 HT(2B), 5 HT2B, HTR2B, Serotonin receptor 2B

验证数据展示

  • WB analysis of mouse brain using 26408-1-AP

    WB analysis of mouse brain using 26408-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26408-1-AP (HTR2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26408-1-AP

    IHC staining of human brain using 26408-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26408-1-AP (HTR2B Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 26408-1-AP

    IHC staining of human brain using 26408-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26408-1-AP (HTR2B Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26408-1-AP targets HTR2B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HTR2B fusion protein Ag24007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5-hydroxytryptamine (serotonin) receptor 2B
Synonyms 5 HT 2B, 5 HT(2B), 5 HT2B, HTR2B, Serotonin receptor 2B
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC063123
Gene Symbol HTR2B
Gene ID (NCBI) 3357
RRIDAB_2880503
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41595
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The serotonin (5-HT) gene has been implicated in the development of personality traits or temperament predisposition. HTR2B(serotonin receptor 2B) has been shown that 3,4-methylene-dioxymethamphetamine, commonly referred to as the drug ecstasy, selectively binds and activates 5-HT2B receptors to induce serotonin release in mouse raphe nuclei, which leads to dopamine release in the nucleus accumbens and ventral tegmentum (PMID:23774082). Moreover, One of the most reliable predictive markers of UM (Uveal melanoma) at risk of evolving toward the formation of liver lesions is an abnormally elevated level of expression of the transcript encoding the HTR2B (PMID:31002821).

实验方案

Product Specific Protocols
WB protocol for HTR2B antibody 26408-1-APDownload protocol
IHC protocol for HTR2B antibody 26408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Hypertension

Subtypes of Histopathologically Classical Aldosterone-Producing Adenomas Yield Various Transcriptomic Signaling and Outcomes.

Authors - Vin-Cent Wu
View Article