验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
22189-1-AP targets HTR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16753 Product name: Recombinant human HTR1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 228-315 aa of BC096207 Sequence: YGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSESGSPVYVNQVKVRVSDALLEKKKLMAARERKATKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5-hydroxytryptamine (serotonin) receptor 1B |
| 别名 | 5 HT 1B, 5 HT 1D beta, 5 HT1B, 5 HT1DB, HTR1B, HTR1D2, HTR1DB, S12, Serotonin 1D beta receptor, Serotonin receptor 1B |
| 计算分子量 | 390 aa, 44 kDa |
| 观测分子量 | 40-44 kDa |
| GenBank蛋白编号 | BC096207 |
| 基因名称 | HTR1B |
| Gene ID (NCBI) | 3351 |
| RRID | AB_2879020 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28222 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HTR1B, also known as 5-HT1B, 5-HT1DB, HTR1D2, HTR1DB, and S12, belongs to the G-protein coupled receptor 1 family. HTR1B is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of HTR1B is mediated by G proteins that inhibit adenylate cyclase activity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HTR1B antibody 22189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse. | ||
Heliyon HTR1B regulates mitochondrial homeostasis and mitophagy by activating the ERK/ MAPK signalling pathway during human embryonic arrest | ||
Eur J Neurosci The alteration of serotonergic markers in the amygdala and raphe nuclei of oestrogen receptor β knock-out female mice |
