• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66723-1-Ig (same clone as 66723-1-PBS)

    WB analysis using 66723-1-Ig (same clone as 66723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66723-1-Ig (HSPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

    IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66723-1-Ig (same clone as 66723-1-PBS)

    IF Staining of HeLa using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66723-1-Ig (HSPH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

HSPH1 Monoclonal antibody, PBS Only

HSPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66723-1-PBS

产品说明书

CloneNo. 3H5A5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66723-1-PBS targets HSPH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 HSPH1 fusion protein Ag27521 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 105kDa/110kDa protein 1
别名 Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25
计算分子量 858 aa, 97 kDa
观测分子量 110 kDa
GenBank蛋白编号BC037553
基因名称 HSPH1
Gene ID (NCBI) 10808
RRIDAB_2882074
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92598
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. Western blot analysis using this antibody detected the phosphorylated protein around 110 kDa in various lysates.