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验证数据展示

  • WB analysis using 16512-1-AP

    WB analysis using 16512-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16512-1-AP (HSP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16512-1-AP

    IHC staining of human lung cancer using 16512-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP10 antibody included
  • IHC staining of human lung cancer using 16512-1-AP

    IHC staining of human lung cancer using 16512-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP10 antibody included

HSP10 Polyclonal antibody

HSP10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16512-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

HSPE1, 10 kDa chaperonin, CPN10, Early-pregnancy factor, Heat shock protein family E member 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2
Positive IHC detected inhuman lung cancer tissue, mouse adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSP10 Ready-To-Use IHC Kit. HSP10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16512-1-AP targets HSP10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性chicken
免疫原 HSP10 fusion protein Ag9732 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 10kDa protein 1 (chaperonin 10)
别名 HSPE1, 10 kDa chaperonin, CPN10, Early-pregnancy factor, Heat shock protein family E member 1
计算分子量 102 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC023518
基因名称 HSP10
Gene ID (NCBI) 3336
RRIDAB_3085493
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61604
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSP10 antibody 16512-1-APDownload protocol
IHC protocol for HSP10 antibody 16512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols