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验证数据展示

  • WB analysis using 16512-1-AP

    WB analysis using 16512-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16512-1-AP (HSP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16512-1-AP

    IHC staining of human lung cancer using 16512-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP10 antibody included
  • IHC staining of human lung cancer using 16512-1-AP

    IHC staining of human lung cancer using 16512-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP10 antibody included

HSP10 Polyclonal antibody

HSP10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16512-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

HSPE1, 10 kDa chaperonin, CPN10, Early-pregnancy factor, Heat shock protein family E member 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2
Positive IHC detected inhuman lung cancer tissue, mouse adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSP10 Ready-To-Use IHC Kit. HSP10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16512-1-AP targets HSP10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性chicken
免疫原

CatNo: Ag9732

Product name: Recombinant human HSPE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC023518

Sequence: MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 10kDa protein 1 (chaperonin 10)
别名 HSPE1, 10 kDa chaperonin, CPN10, Early-pregnancy factor, Heat shock protein family E member 1
计算分子量 102 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC023518
基因名称 HSP10
Gene ID (NCBI) 3336
RRIDAB_3085493
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61604
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSP10 antibody 16512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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