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验证数据展示

  • WB analysis using 83466-1-RR (same clone as 83466-1-PBS)

    WB analysis using 83466-1-RR (same clone as 83466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83466-1-RR (HSPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83466-1-RR (same clone as 83466-1-PBS)

    WB analysis of HeLa using 83466-1-RR (same clone as 83466-1-PBS)

    WB result of HSPA2 antibody (83466-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells. This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00465-1

    Sandwich ELISA standard curve of MP00465-1

    Sandwich ELISA standard curve of MP00465-1, Human HSPA2 Recombinant Matched Antibody Pair - PBS only. 83466-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3539. 83466-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP00465-1

    Sample test of MP00465-1

    The mean HSPA2 concentration was determined to be 34.57 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

  • IHC staining of mouse testis using 83466-1-RR (same clone as 83466-1-PBS)

    IHC staining of mouse testis using 83466-1-RR (same clone as 83466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
  • IHC staining of mouse testis using 83466-1-RR (same clone as 83466-1-PBS)

    IHC staining of mouse testis using 83466-1-RR (same clone as 83466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA2 antibody included
  • Cytometric bead array standard curve of MP00465-1

    Cytometric bead array standard curve of MP00465-1

    Cytometric bead array standard curve of MP00465-1, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-3-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00465-2

    Cytometric bead array standard curve of MP00465-2

    Cytometric bead array standard curve of MP00465-2, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-2-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83466-1-RR

    Affinity and Kinetic Characterization of 83466-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83466-1-RR against Human HSPA2 were performed. The affinity constant is 1.86 nM.

  • Featured Product
  • KD/KO验证

HSPA2 Recombinant antibody, PBS Only (Capture)

HSPA2 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83466-1-PBS

产品说明书

CloneNo. 240421A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSPA2 Ready-To-Use IHC Kit. HSPA2 primary antibody included.

产品信息

83466-1-PBS targets HSPA2 as part of a matched antibody pair:

MP00465-1: 83466-1-PBS capture and 83466-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00465-2: 83466-1-PBS capture and 83466-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 HSPA2 fusion protein Ag3539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock 70kDa protein 2
别名 HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2
计算分子量 639 aa, 70 kDa
观测分子量70 kDa
GenBank蛋白编号BC036107
基因名称 HSPA2
Gene ID (NCBI) 3306
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54652
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)