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验证数据展示

  • WB analysis using 25405-1-AP (same clone as 25405-1-PBS)

    WB analysis using 25405-1-AP (same clone as 25405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 25405-1-AP (same clone as 25405-1-PBS)

    IP experiment of HEK-293 using 25405-1-AP (same clone as 25405-1-PBS)

    IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:5000) with HEK-293 cells lysate 2320 ug. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 25405-1-AP (same clone as 25405-1-PBS)

    IHC staining of human tonsillitis using 25405-1-AP (same clone as 25405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25405-1-AP (HSP70 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 25405-1-AP (same clone as 25405-1-PBS)

    IHC staining of human stomach using 25405-1-AP (same clone as 25405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25405-1-AP (HSPA1B antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 25405-1-AP (same clone as 25405-1-PBS)

    IF Staining of HeLa using 25405-1-AP (same clone as 25405-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HSPA1B antibody (25405-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

HSPA1B Polyclonal antibody, PBS Only

HSPA1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25405-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

HSP70, HSP72

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25405-1-PBS targets HSPA1B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13700

Product name: Recombinant human HSPA1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-641 aa of BC057397

Sequence: DFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 70kDa protein 1B
别名 HSP70, HSP72
计算分子量 70 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC057397
基因名称 HSP70
Gene ID (NCBI) 3304
RRIDAB_2880063
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08107
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1. What is Hsp70/HSP1A?

HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.


2. FAQs for Hsp70

a. I cannot detect Hsp70 by western blotting

HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

a. What loading control can I use for cellular stress experiments with Hsp70?

Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.


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