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验证数据展示

  • WB analysis using 19016-1-AP

    WB analysis using 19016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19016-1-AP (HSPA12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 19016-1-AP

    IP experiment of mouse brain using 19016-1-AP

    IP result of anti-HSPA12B (IP:19016-1-AP, 4ug; Detection:19016-1-AP 1:800) with mouse brain tissue lysate 1840 ug.

HSPA12B Polyclonal antibody

HSPA12B Polyclonal Antibody for WB, IP, ELISA
Cat No. 19016-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Heat shock protein family A member 12B, heat shock 70kD protein 12B, Heat shock 70 kDa protein 12B, C20orf60

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse lung tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19016-1-AP targets HSPA12B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HSPA12B fusion protein Ag13544 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 70kD protein 12B
别名 Heat shock protein family A member 12B, heat shock 70kD protein 12B, Heat shock 70 kDa protein 12B, C20orf60
计算分子量 686 aa, 76 kDa
观测分子量 75 kDa
GenBank蛋白编号BC110881
基因名称 HSPA12B
Gene ID (NCBI) 116835
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MM6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSPA12B antibody 19016-1-APDownload protocol
IP protocol for HSPA12B antibody 19016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols